Genetic polymorphisms in the promoter region of cyclooxygenase-2 and their association with risk of gastric cancer.
- Author:
Xue-mei ZHANG
1
;
Xiao-ping MIAO
;
Wen TAN
;
Tong SUN
;
Yong-li GUO
;
Dan ZHAO
;
Dong-xin LIN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Cyclooxygenase 2; genetics; Female; Genetic Predisposition to Disease; Haplotypes; Humans; Male; Middle Aged; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; genetics; Risk; Stomach Neoplasms; enzymology; genetics
- From: Acta Academiae Medicinae Sinicae 2006;28(2):119-123
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine the genetic polymorphisms in the promoter region of cyclooxygenase-2 ( COX-2) and evaluate their association with the risk of gastric cancer.
METHODSSingle-strand conformational polymorphism and DNA sequencing were used to screen the genetic variants of the COX-2 promoter region. Total 323 patients with gastric cancer and 646 frequency-matched controls were genotyped by polymerase chain reaction-restriction fragment length polymorphism method. Odds ratios (OR) and 95% confidence intervals (CI) were estimated by logistic regression. Haplotype frequency was estimated using Haplo. stat software.
RESULTSThree single nucleotide polymorphisms, including - 1290A > G, -1195G > A, and -765G > C were identified in the promoter of COX- 2. Case-control analysis showed an increased risk of developing gastric cancer for the -1290AG (OR 1.64; 95% CI 1.03-2.61), -1195AA (OR 2.68; 95% CI 1.65-4.33), and -765CG (OR 2.66; 95% CI 1.53-4.64) genotype carriers, respectively, compared with noncarriers. A greater risk of developing gastric cancer was observed for the A(-1290) -A(-1195) -C(-765) haplotype compared with the A(-1290) -G(-1195) -G(-765) haplotypes (OR 11.80; 95% CI 2.43-57.25).
CONCLUSIONGenetic polymorphisms in COX-2 promoter region may play a role in gastric carcinogenesis.
