Association analysis of 30 type 2 diabetes candidate genes in Chinese Han population.
- Author:
Zhuo LIU
1
;
Yong-wei ZHANG
;
Qi-ping FENG
;
Yun-feng LI
;
Guo-dong WU
;
Jin ZUO
;
Xin-hua XIAO
;
Fu-de FANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Asian Continental Ancestry Group; genetics; Case-Control Studies; Diabetes Mellitus, Type 2; genetics; Female; Genetic Predisposition to Disease; Genetic Testing; Genotype; Humans; Male; Middle Aged; Polymorphism, Single Nucleotide
- From: Acta Academiae Medicinae Sinicae 2006;28(2):124-128
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the susceptibility genes of type 2 diabetes in Chinese Han population.
METHODSSingle nucleotide polymorphism (SNP) discovery, genotyping and haplotype construction were performed in 30 candidate genes. Case-control study were carried out in a population-based sample and confirmed by the transmission disequilibrium test (TDT) analysis in 77 trio pedigrees. The effects of the SNP rs5210 on gene expression were studied by reporter gene technique.
RESULTSThe case-control studies showed that several SNPs on KCNJ11 gene was associated with type 2 diabetes in Chinese Han population, in which the allele frequency of SNP rs5219, the genotype frequency of rs5210, rs2285676 and rs5219, and the frequency of haplotype GA combined of the rs5219 and rs5215 showed significant difference between these two groups (P < 0.05). In addition, TDT test also showed statistical significance on this haplotype GA (P < 0. 05). The reporter gene assay showed that the effect on gene expression was significantly different between two alleles of rs5210 (P < 0.05).
CONCLUSIONKCNJII gene is one of the susceptibility genes of type 2 diabetes in Chinese Han population.
