Dystrophin expression in mdx mice after bone marrow stem cells transplantation.
- Author:
Shan-wei FENG
1
;
Cheng ZHANG
;
Xiao-li YAO
;
Mei-juan YU
;
Jing-lun LI
;
Song-lin CHEN
;
Tai-yun LIU
;
Xi-lin LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; metabolism; Cell Differentiation; Disease Models, Animal; Dystrophin; biosynthesis; Hematopoietic Stem Cell Transplantation; Male; Mice; Mice, Inbred C57BL; Mice, Inbred mdx; Muscular Dystrophy, Duchenne; metabolism; surgery; Transplantation, Homologous
- From: Acta Academiae Medicinae Sinicae 2006;28(2):178-181
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the dynamic changes of dystrophin expression in mdx mice after bone marrow stem cells transplantation.
METHODSThe bone marrow stem cells of C57 BL/6 mice (aged 6 to 8 weeks) were injected intravenously into the mdx mice (aged 7 to 9 weeks), which were preconditioned with 7Gy gamma ray. The amount of dystrophin;expression in gastrocnemius was detected by immunofluorescence, reverse transcription-polymerase chain reaction and Western blot at week 5, 8, 12 and 16 after transplantation.
RESULTSAt week 5 after bone marrow stem cells transplantation, the dystrophin expression detected in mdx mice were very low; however, its expression increased along with time. At week 16 week, about 12% muscle cells of all transplanted mice expressed dystrophin. There were less centrally placed myonuclei than the control mdx mice, whereas peripheral myonuclei increased.
CONCLUSIONSAfter having been injected into mdx mice, the allogenic bone marrow stem cells have a trend to reach the injured muscle tissues and differentiate to fibers that can express dystrophin and the expression increased with time. The bone marrow stem cells participates in the repair and regeneration of the injured tissues permanently and constantly.
