Effect of combination of glutamine and mitomycin C on human gastric carcinoma cell strain MGC-803.
- Author:
Wei-Zhao PENG
1
;
Jian-Chun YU
;
Yu-Qin LIU
;
Wei-Ming KANG
;
Yue-Ping FAN
;
Wei-Dong GUO
;
Qun ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; pathology; Antibiotics, Antineoplastic; pharmacology; Cell Line, Tumor; Dose-Response Relationship, Drug; Drug Synergism; Glutamine; pharmacology; Humans; Mitomycin; pharmacology; Stomach Neoplasms; pathology
- From: Acta Academiae Medicinae Sinicae 2006;28(3):345-349
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of combination of glutamine (GLN) and mitomycin C (MMC) on the human gastric carcinoma cell line MGC-803 in vitro.
METHODSThe effects of GLN and MMC were measured by MTT assay, and the interaction between the two agents was evaluated by the median-effect principle. Flow cytometry was used for cell cycle analysis.
RESULTSGLN did not significantly stimulate the cell growth in vitro. High-concentration of GLN could inhibit the cell growth. MMC could effectively inhibit the cell growth in a time-dependent manner. The interaction of these two agents showed a weak antagonistic activity (1 < CI < 1.2703). MMC induced remarkable S-phase arrest. Low-dose GLN has limited effect on the S-phase arrest of MMC, while high-dose GLN significantly attenuated the S-phase arrest and lowered the proliferation index of MGC-803 cell.
CONCLUSIONSCombination of GLN and MMC has a a weak and dose-dependent antagonistic activity in the treatment of gastric carcinoma cell line MGC-803. The combination of high-dose MMC and low-dose GLN may achieve better efficacy.
