Hemangiopoiet in modulates adhesive properties of endothelial cells.
- Author:
Xiao-Jing WANG
1
;
Yong-Jun LIU
;
Shi-Hong LU
;
Lin-Hui LIANG
;
Han WANG
;
Zhong-Chao HAN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Adhesion Molecules; metabolism; Cells, Cultured; E-Selectin; biosynthesis; genetics; Endothelial Cells; cytology; Humans; Intercellular Adhesion Molecule-1; biosynthesis; genetics; Proteoglycans; pharmacology; RNA, Messenger; biosynthesis; genetics; Umbilical Veins; cytology; Vascular Cell Adhesion Molecule-1; biosynthesis; genetics
- From: Acta Academiae Medicinae Sinicae 2006;28(3):364-367
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of hemangiopoietin (HAPO) on the adhesive properties of human umbilical vein endothelial cells (HUVEC).
METHODSThe adhesion of HUVEC and the expressions of CD54, CD102, CD106, CD31, CD62E, and CD62P were measured by adhesion assay, flow cytometry, and semi-quantitative RT-PCR.
RESULTSHAPO enhanced the total adherence of HUVEC in a concentration-dependent manner. Flow cytometry analysis revealed that the treatment of HAPO resulted in a significantly increased expression of CD106 and CD62E on HUVEC in a time-dependent manner. When HUVEC were incubated with HAPO for 6 h, the percentage of CD106 + HUVEC and CD62E HUVEC increased about 2.10 folds and 5.84 folds, respectively, compared with control. The time-course of adhesive molecules mRNA expression indicated that the expression of CD106 and CD62E reached at the maximum 1.86 folds and 6.16 folds, respectively, compared with control.
CONCLUSIONHAPO may facilitate the homing of hematopoietic stem/progenitor cells.
