Toxic effect of butenolide on chondrocyte differentiation and the protective effect of selenium.
- Author:
Hong ZUO
1
;
Xiong GUO
;
Shi-Jie WANG
;
Zhong-Li SHI
;
Shuang-Qing PENG
;
Jun-Ling CAO
;
Zeng-Tie ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: 4-Butyrolactone; analogs & derivatives; pharmacology; Cell Differentiation; Cells, Cultured; Chondrocytes; cytology; Humans; Protective Agents; pharmacology; Selenium; pharmacology; T-2 Toxin; toxicity
- From: Acta Academiae Medicinae Sinicae 2006;28(3):382-385
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of butenolide (BUT) on cultured chondrocytes differentiation and the possible protective effects of selenium (Se).
METHODSEx-vivo cultured chondrocytes were divided into six groups: (1) Control group (without BUT and Se); (2) Se 0.1 microg/ml control group; (3) BUT 0.1 microg/ml group; (4) BUT 1.0 microg/ml group; (5) BUT 5.0 microg/ml group; and (6) BUT 1.0 microg/ml + Se 0.1 microg/ml group. The expression of collagen II (Col II), collagen X (ColX), basic fibroblast growth factor (bFGF), and parathyroid hormone-related peptide (PTHrP) in (or around) chondrocytes in all groups were analyzed by immunohistochemistry.
RESULTSThe expressions of Col II in 1.0 microg/ml BUT group and 5.0 microg/ml BUT group were significantly lower than those in the control group (P < 0.05). The expression of Col II in 1.0 microg/ml BUT + Se group were significantly higher than those in the 1.0 microg/ml BUT group and 5.0 microg/ml BUT group (P < 0.05). The expressions of bFGF and PTHrP of BUT groups were significantly higher than those in the Se and control groups (P < 0.05). No expression of ColX was observed in all groups.
CONCLUSIONBUT can affect the collagen II synthesis of the chondrocytes. Selenium supplementation may play a protective role.
