Determination content of baicalin in Yinhuang granules from different pharmaceutical factories by near-infrared spectroscopy.
- Author:
Yan BAI
1
;
Wei ZHANG
;
Xing WANG
Author Information
- Publication Type:Journal Article
- MeSH: Drugs, Chinese Herbal; chemistry; Flavonoids; analysis; Quality Control; Spectroscopy, Near-Infrared; methods
- From: China Journal of Chinese Materia Medica 2010;35(2):166-168
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the content of baicalin in Yinhuang granules fastly from different pharmaceutical factories with near-infrared spectroscopy.
METHODNear-infrared diffuse reflectance spectroscopy technology was used to collect NIR spectra of Yinhuang granules. With HPLC anilysis values as reference, the fast determination method of baicalin was established with partial least squares (PLS).
RESULTThe correlation coefficients (R2), the root-mean-square error of cross-validation and the root-mean-square error of calibration (RMSEC) of the calibration model for baicalin were 0.9982, 0.1899 and 0.0514, respectively; the root-mean-square error of prediction (RMSEP) and the average rate of recovery were 0.0802 and 104.27%.
CONCLUSIONThe method is accurate, fast and simple, and can be applied for the fast determination of large quantities of numbers of samples.
