Simultaneous determination of three active compounds in root barks of Dictamnus dasycarpus by RP-HPLC.
- Author:
Xiaojuan YANG
1
;
Yanfang LIU
;
Zhong BAO
;
Yong JIANG
;
Pengfei TU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Chromatography, Reverse-Phase; methods; Dictamnus; chemistry; Plant Bark; chemistry; Plant Extracts; analysis; Plant Roots; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(2):187-190
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a RP-HPLC method for simultaneous determination of three active compounds, dictamnine, obacunone and fraxinellone in root bark of Dictamnus dasycarpus and supply a reference for the establishment of the quality standard of D. dasycarpus.
METHODA Kromasil C18 column was used with methanol-water (60:40) as the mobile phase, at the flow rate of 1 mL x min(-1). 236 nm was selected as the detected wavelength.
RESULTThe determined three compounds were well separated with a linear range of 0.0021-0.1060, 0.0201-0.9200 and 0.0102-1.020 g x L(-1), respectively. The recoveries of them were 100.5%, 99.2% and 100.2%, respectively.
CONCLUSIONThis method is simple, rapid and accurate, particularly suitable for the quality control of D. dasycarpus.
