Prophylactic protective effects of paeoniflorin on corticosterone-induced primary cultures of rat cortical neurons damages.
- Author:
Yang LIU
1
;
Guangzhi CUI
;
Yanjun ZHANG
;
Xiumei GAO
;
Yan GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Benzoates; pharmacology; Bridged-Ring Compounds; pharmacology; Cell Proliferation; drug effects; Cells, Cultured; Cerebral Cortex; cytology; Corticosterone; pharmacology; Female; Glucosides; pharmacology; Male; Monoterpenes; Neurons; cytology; drug effects; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2010;35(2):208-210
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the prophylactic neuroprotective effect of paeoniflorin (PF) on corticosterone-induced primary cultures of rat cortical neurons damages.
METHODPrimary cultures of neurons from the of 16-days-old embryo rats were used to study. Neurons in the experimental group were treated with cort (200 micromol x L(-1)) 30 min after the pretreatment of PF (0.5, 2, 10 micromol x L(-1)). We used MTT assay to detect the action of paeoniflorin on neuronal activity. DAPI staining to study of the influence of PF on neuronal apoptosis morphology, and Annexin V-FITC/PI apoptosis detection kit to inspect the impact of paeoniflorin on the early neuronal apoptosis.
RESULTCompared with model group, every dose of PF (0.5, 2, 10 micromol x L(-1)) can raise the neuronal activity notably (P<0.05), PF of 2 micromol x L(-1) and 10 micromol x L(-1) can reduce the rate of neuronal apoptosis.
CONCLUSIONOur data indicated that PF achieved prophylactic neuroprotective effect through against corticosterone-induced neurons damages.
