Anti-tumor activity of safflower polysaccharide (SPS) and effect on cytotoxicity of CTL cells, NK cells of T739 lung cancer in mice.
- Author:
Xuekui SHI
1
;
Dianqing RUAN
;
Yaxian WANG
;
Li MA
;
Mingqi LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; administration & dosage; Carthamus tinctorius; chemistry; metabolism; Cell Line, Tumor; Disease Models, Animal; Female; Humans; Killer Cells, Natural; immunology; Lung Neoplasms; drug therapy; immunology; pathology; Male; Mice; Mice, Inbred BALB C; Plant Extracts; administration & dosage; Polysaccharides; administration & dosage; T-Lymphocytes, Cytotoxic; immunology; Tumor Burden; drug effects
- From: China Journal of Chinese Materia Medica 2010;35(2):215-218
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the anti-tumor activity of SPS in vivo and in vitro and the cytotoxicity of CTL cells, NK cells of T739 lung cancer in mice.
METHODThe transplanted tumor model of S180 Sarcoma was established with KM mouse. The SPS was adminished i.p. for 10 d, the tumor weight was detected. The transplanted tumor model of LA795 lung cancer was established with T739 mouse and SPS was adminished i.p. for 10 d and the tumor weight and the cytotoxicity of CTL cells, NK cells were detected. The Anti-tumor activity of SPS on three types of tumor cells in vitro was observed with trypan blue exclusion staining.
RESULTSPS 40 mg x kg(-1) can significantly inhibit the growth of S180 Sarcoma in mice and inhibitory rate was 51.33% (P<0.01). It can also inhibit the growth of LA795 lung cancer in mice and the tumor volume was reduced obviously for 3.29 mm3 (P<0.05). It can remarkably enhance the cytotoxicity of splenic CTL cells, NK cells in tumor-bearing (P<0.05).
CONCLUSIONSPS have anti-tumor effects, the mechanism of the anti-tumor activity may be related to enhance the cytotoxicity of CTL cell and NK cell.
