Determination of paprazine in Polygonum orientale of different commodities by HPLC.
- Author:
Jie SONG
1
;
Yanjun ZHAI
;
Rongxiang WANG
;
Na LI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; chemistry; Polygonum; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(3):272-274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for the content determination of paprazine in Polygonum orientale, and provide a new evaluation index for the quality control for P. orientale.
METHODHPLC was carried out on Agilent C18 (4.6 mm x 150 mm, 5 microm) with methanol-water (40:60) as mobile phase. The flow rate was 1.0 mL x min(-1) and the wavelength of UV detector was set at 290 nm.
RESULTThe calibration curve was linear in the range of 0. 038-0.190 microg (r = 0.9999). The average recovery was 98.7% with the RSD of 2.36%.
CONCLUSIONThe method appears to be simple, accurate and reproducible, it can be used to determine the content of paprazine in P. orientale.
