Polysaccharides from Scurrula parasitica L. inhibit sarcoma S180 growth in mice.
- Author:
Yijun XIAO
1
;
Yanli FAN
;
Binghua CHEN
;
Qiujin ZHANG
;
Hong ZENG
Author Information
1. College of Life Sciences, Fujian Normal University, Fuzhou 350108, China. xiaoyijun@fjnu.edu.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Cyclin D1;
metabolism;
Drugs, Chinese Herbal;
chemistry;
therapeutic use;
Female;
HL-60 Cells;
Humans;
Immunohistochemistry;
K562 Cells;
Loranthaceae;
chemistry;
Male;
Mice;
Plants, Medicinal;
chemistry;
Polysaccharides;
therapeutic use;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Sarcoma 180;
drug therapy;
metabolism;
bcl-2-Associated X Protein;
metabolism
- From:
China Journal of Chinese Materia Medica
2010;35(3):381-384
- CountryChina
- Language:Chinese
-
Abstract:
To study the anti-tumor activity of Scurrula parasitica polysaccharides (SP). Water extraction and ethanol precipitation were used to isolate SP from S. parasitica leaf. S180, K562 and HL-60 cell lines proliferation inhibition by SP were detected by MTT assay. The expressions of Ki-67, Cyclin D1, Bax and Bcl-2 protein in the sarcoma S180 tissues were detected by immunohistochemistry technique to approach the anti-tumor mechanism of SP+ SP could not inhibit cancer cell proliferation. SP ip could inhibit the growth of sarcoma S180 in mice, 100 mg x kg(-1) x d(-1). SP ip was the optimal dose on inhibiting S180 growth, with the tumor inhibition rate of 54%. The expression of Ki-67, Cyclin D1, Bax and Bcl-2 protein in the sarcoma S180 tissues were detected by immunohistochemistry technique to approach the anti-tumor mechanism of SP. The result showed that SP could down-regulate the expression of Ki-67, CyclinD1 and Bcl-2 protein, and up-regulate the expression of Bax protein. It indicted that inhibiting cancer cell proliferation and promoting cancer cell apoptosis in vivo maybe one of the anti-cancer mechanisms of SP.
