Research progress of whole embryo culture tool and its application.
- Author:
Jiayin HAN
1
;
Aihua LIANG
Author Information
1. Capital Medical University, School of Traditional Chinese Medicine, Beijing 100069, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Culture Media;
Embryo Culture Techniques;
methods;
Embryonic Development;
drug effects;
Environmental Pollutants;
toxicity;
Metals, Heavy;
toxicity;
Mice;
Rats
- From:
China Journal of Chinese Materia Medica
2010;35(5):549-553
- CountryChina
- Language:Chinese
-
Abstract:
Whole embryo culture (WEC) is an experimental tool, which is made use of embryos in vitro to replace whole animals to investigate the growth and development of early organs, the embryo toxicity of chemical materials and the mechanism of the occurrence of embryo toxicity. Compared with experiment with whole animals, WEC could reduce the number of experimental animals, shorten experimental time, decrease experimental expenses, eliminate disturbing factors and control dosage more exactly. So it is generally received that WEC tool is a good experimental method to match the principles of replacement, reduction, refinement and responsibility. This article is a review of the WEC tool of rat and mouse, including the development of this tool, announcements, and the application in the development of organs, the embryo toxicity of environmental pollution and heavy metal, safety evaluation of medicine and the embryo toxicity of traditional Chinese medicine and its mechanism. There is also a discussion of the application of this tool in the investigation of the embryo toxicity of traditional Chinese medicine.
