Inhibitory effects of lovastatin on the proliferation and cell cycle phase of cultured human glomerular mesangial cells in vitro.
- Author:
Hang LI
1
;
Xue-wang LI
;
Lin DUAN
;
Chen-hong LI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Cycle; drug effects; Cell Division; drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Flow Cytometry; Glomerular Mesangium; cytology; Humans; Hydroxymethylglutaryl-CoA Reductase Inhibitors; pharmacology; Kinetics; Lovastatin; pharmacology
- From: Acta Academiae Medicinae Sinicae 2002;24(1):63-66
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects and mechanism of lovastatin on cell cycle phase and proliferation of cultured human glomerular mesangial cells in vitro.
METHODSHMC proliferation was determined by 3H-Thymidine incorporation. HMC cell cycle was measured by flow cytometric analysis.
RESULTSLovastatin was found to inhibit HMC proliferation in a dose-dependent manner. Flow cytometric analysis demonstrated that lovstatin induced G1/S transition arrest. Concomitant addition of mevalonate or farnesol restored all the inhibitory effect of lovstatin on HMC.
CONCLUSIONLovastatin is a HMC proliferation inhibitor. It provides an experimental evidence for re-evaluate renal protective effect of HRI, which has already been widely used in clinical treatment.
