Effects of seawater immersion on the functions of mitochondria of myocardium and hepatocyte in hemorrhagic shock rats.
- Author:
Song-min LU
1
;
Jian-cang LIU
;
Cheng-he SHI
;
Ping LI
;
Qing-jun LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium; metabolism; Immersion; Male; Mitochondria, Heart; enzymology; Mitochondria, Liver; enzymology; Proton-Translocating ATPases; metabolism; Random Allocation; Rats; Rats, Wistar; Seawater; Shock, Hemorrhagic; enzymology; physiopathology
- From: Chinese Journal of Traumatology 2006;9(1):38-42
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of seawater immersion on the function of myocardium and hepatocyte mitochondria in experimental hemorrhagic shock rats.
METHODSTwenty-four male Wistar rats were divided into three groups (n=8 in each group): control group, HSL group (hemorrhagic shock group on land) and HSS group (hemorrhagic shock group in seawater). The hemodynamic parameters, activities of H(+)-ATPase (adenosinetriphosphatase), succinate dehydrogenase (SDH) and Ca(2+)-Mg(2+)-ATPase, the calcium contents in myocardium and hepatocyte mitochondria were measured and the changes of proton translocation across the inner mitochondrial membrane were analyzed.
RESULTSThe hemodynamic indexes and the activities of H+-ATPase, SDH, Ca(2+)-Mg(2+)-ATPase in HSS group were significantly lower than those in control group and HSL group (P<0.05). In HSS group the calcium levels in tissue and mitochondria of myocardium and hepatocyte were elevated significantly compared with control group and HSL group (P<0.05). There was no significant difference in proton translocation among three groups.
CONCLUSIONSThis investigation demonstrates that seawater immersion can aggravate the conditions of hemorrhagic shock rats.
