The experimental study of genetic engineering human neural stem cells mediated by lentivirus to express multigene.
- Author:
Pei-qiang CAI
1
;
Xun TANG
;
Yue-qiu LIN
;
Oudega MARTIN
;
Guang-yun SUN
;
Lin XU
;
Yun-kang YANG
;
Tian-hua ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; Cells, Cultured; Feasibility Studies; Gene Expression; Genetic Engineering; methods; Genetic Therapy; methods; Genetic Vectors; genetics; Green Fluorescent Proteins; Humans; Immunohistochemistry; Lentivirus; genetics; Microscopy, Fluorescence; Neurons; metabolism; Rats; Stem Cell Transplantation; Stem Cells; metabolism; Transgenes
- From: Chinese Journal of Traumatology 2006;9(1):43-49
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the feasibility to construct genetic engineering human neural stem cells (hNSCs) mediated by lentivirus to express multigene in order to provide a graft source for further studies of spinal cord injury (SCI).
METHODSHuman neural stem cells from the brain cortex of human abortus were isolated and cultured, then gene was modified by lentivirus to express both green fluorescence protein (GFP) and rat neurotrophin-3 (NT-3); the transgenic expression was detected by the methods of fluorescence microscope, dorsal root ganglion of fetal rats and slot blot.
RESULTSGenetic engineering hNSCs were successfully constructed. All of the genetic engineering hNSCs which expressed bright green fluorescence were observed under the fluorescence microscope. The conditioned medium of transgenic hNSCs could induce neurite flourishing outgrowth from dorsal root ganglion (DRG). The genetic engineering hNSCs expressed high level NT-3 which could be detected by using slot blot.
CONCLUSIONSGenetic engineering hNSCs mediated by lentivirus can be constructed to express multigene successfully.
