Nude mice intraperitoneally transfused with ex vivo expanded bone marrow CD34+ CD59+ cells from patients with paroxysmal nocturnal hemoglobinuria.
- Author:
Yu-Ping ZHONG
1
;
Yong-Ji WU
;
Ti SHEN
;
Xuan WANG
;
Jie-Ping ZHANG
Author Information
1. Department of Hematology, Beijing Chaoyang Hospital Affiliated of Capital University of Medical Sciences, Bejing 100043, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antigens, CD34;
analysis;
Bone Marrow Cells;
cytology;
immunology;
CD59 Antigens;
analysis;
Female;
Hematopoietic Stem Cell Transplantation;
methods;
Hemoglobinuria, Paroxysmal;
pathology;
therapy;
Humans;
Immunomagnetic Separation;
Male;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Transplantation, Heterologous
- From:
Journal of Experimental Hematology
2006;14(1):75-78
- CountryChina
- Language:Chinese
-
Abstract:
Ex vivo expanded human bone marrow CD34(+)CD59(+) cells from patients with paroxysmal nocturnal hemoglobinuria (PNH) were transplanted into BALB/c mice in order to investigate their proliferation ability and reconstruction of hemopoiesis, and to lay the groundwork for clinical ABMT/APBSCT in PNH patients. CD34(+)CD59(+) cells were selected from the bone marrow mononuclear cells in PNH patients by using immunomagnetic positive double sorting. Sublethally irradiated BALB/c mice were transplanted with CD34(+)CD59(+) cells enriched from bone narrow of PNH patients. The results showed that human CD45(+) cells were detected in the bone marrow, spleen and peripheral blood of the nude mice by flow cytometry and DNA analysis at 6 weeks post-transplant. Blood routine indicators of nude mice were found to recover to some extent, but did not fully recover. It is concluded that ex vivo expanded bone marrow CD34(+)CD59(+) cells from patients with paroxysmal nocturnal hemoglobinuria could keep their biological characteristics and ability to reconstruct hemopoiesis in irradiated BALB/c mice.
