Proliferation of natural killer T cells in umbilical cord blood and peripheral blood and their different phenotypes.
- Author:
Yan LIU
1
;
Hua-Hua FAN
;
Ming RUAN
;
Li GAO
;
Xiao-Xuan NIE
;
Yi-Ming YANG
;
Hua-Zhong LU
;
Feng GAO
Author Information
1. Department of Blood Engineering, Shanghai Blood Center, Shanghai 200051, China. yayaliu117@163.com
- Publication Type:Journal Article
- MeSH:
Cell Proliferation;
Cells, Cultured;
Fetal Blood;
cytology;
Galactosylceramides;
pharmacology;
Humans;
Interleukin-2;
pharmacology;
Killer Cells, Natural;
cytology;
drug effects;
immunology;
Leukocytes, Mononuclear;
cytology;
Phenotype;
T-Lymphocytes, Regulatory;
cytology;
drug effects;
immunology
- From:
Journal of Experimental Hematology
2006;14(1):128-132
- CountryChina
- Language:Chinese
-
Abstract:
Purpose of this study was to establish an effective method in vitro to proliferate natural killer T (NKT) cells from umbilical cord blood (UCB) and peripheral blood (PB), and to study their different phenotype. Mononuclear cells (MNC) from UCB and PB were cultured in the presence of IL-2 (100 U/ml), with or without alpha-Galcer. TCR Valpha24 Vbeta11 double positive natural killer T-cells (NKT cells) and their other phenotypes were determined by flow cytometry. The results showed that after expansion for 7 days, TCRValphabeta(+) NKT cells from UCB-MNCs increased by (8.74 +/- 4.37) x 10(2) times as much, but most of them did not express NK1.1 and its TCR Vbeta11(+) was higher than TCR Valpha24(+). After expansion for 14 days, TCR Valphabeta(+) NKT cells from PB-MNCs increased by (3.72 +/- 2.01) x 10(2) times, the expression of NK1.1 was high and its TCR Vbeta11(+) was almost equal to TCR Valpha24(+). It is concluded that human TCR Valpha24 Vbeta11 double positive NKT cells can expand by addition of alpha-Galcer. The proliferation efficiency in UCB-MNCs is greater than that in PB-MNCs. Most of the UCB-NKT is NK1.1(-), while the PB-NKT is NK1.1(+), a new subset of NKT cells.
