Analysis of LRP16 gene promoter activity.
- Author:
Xue-Chun LU
1
;
Fang-Ding LOU
;
Wei-Dong HAN
;
Xu-Dong ZHU
;
Yim-Ming MU
;
Zhou-Min XU
;
Li YU
Author Information
1. Department of Gerontological Hematology, General Hospital of PLA, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Chromosomes, Human, Pair 11;
Gene Expression;
Humans;
Luciferases;
metabolism;
Molecular Sequence Data;
Neoplasm Proteins;
biosynthesis;
genetics;
Promoter Regions, Genetic;
genetics;
Sequence Analysis, DNA
- From:
Journal of Experimental Hematology
2006;14(1):146-149
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to analyze the characteristics of LRP16 gene promoter and its activity in order to explore the possible regulation mechanism of LRP16 gene expression. A 2.6 kb genomic DNA sequence of LRP16 5'-end was obtained from NCBI by BLAST software. The 7 target sequences between 0.2 - 2.6 kb from a healthy blood donor DNA sample were amplified by PCR, then identified by DNA sequencing and semi-nest PCR. The verified sequences were analyzed on-line. The results showed that the 7 target sequences were about 400 bp different from each other. All 7 sequences were the same to these GenBank described. At last, all 7 promoter sequences were ligated with luciferase vector, and then the luciferase activity was analyzed in HeLa cells. A known gene promoter sequence can be freely obtained from NCBI database. It is concluded that LRP16 promoter is a standard type II promoter and its activity is strongest in the region from -200 to -600 bp.
