Effect of diallyl disulfide on expression and secretion of VEGF in HL-60 leukemic cells.
- Author:
Yi XIE
1
;
Zi-Li FAN
;
Chen-Jiao YAO
;
San-Qin TAN
;
Ya-Li ZHAO
Author Information
1. Center of Oncology, Changsha Central Hospital, Changsha 410004, China.
- Publication Type:Journal Article
- MeSH:
Allyl Compounds;
pharmacology;
Antineoplastic Agents;
pharmacology;
Cell Proliferation;
drug effects;
Disulfides;
pharmacology;
HL-60 Cells;
Humans;
RNA, Messenger;
biosynthesis;
genetics;
Vascular Endothelial Growth Factor A;
biosynthesis;
genetics
- From:
Journal of Experimental Hematology
2006;14(2):212-216
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to investigate the expression of VEGF mRNA and VEGF protein in HL-60 cells treated with diallyl disulfide (DADS), and to explore the antileukemic mechanism of DADS in respect of VEGF production. Semi-quantitative RT-PCR and ELISA were used to detect the expression of VEGF mRNA and secretion of VEGF protein in HL-60 cell lines treated by DADS respectively. The results showed that the expression of VEGF mRNA and secretion of VEGF protein were found in HL-60 cells. The expression of VEGF mRNA and secretion of VEGF protein in HL-60 cells could be down regulated by treatment with 0.625, 1.25, and 2.5 microg/mL DADS for 48 and 72 hours and the effects had a dose dependent relationship (r > 0.9, P < 0.01). The differences between DADS treated HL-60 cell groups and the control group were statistically significant (P < 0.01), there were also statistically significant differences among three DADS-treated HL-60 cell groups (P < 0.05). It is concluded that DADS effectively inhibits the proliferation of human leukemia cell line HL-60 cells; DADS exerts its antileukemic effects by reduction of the expression of VEGF mRNA and VEGF protein secretion.
