Detection of fusion genes associated with specific translocations in acute leukemia patients with normal karyotypes by using multiplex RT-PCR.
- Author:
Li MA
1
;
Yong-Quan XUE
;
Jin-Lan PAN
;
Jun HE
;
Ya-Fang WU
;
Jian-Nong CEN
;
Bin-Zhao WEN
Author Information
1. Department of Hematology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
- Publication Type:Journal Article
- MeSH:
Acute Disease;
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Cytogenetic Analysis;
Female;
Humans;
Karyotyping;
Leukemia;
genetics;
Leukemia, Myeloid, Acute;
genetics;
Male;
Middle Aged;
Oncogene Proteins, Fusion;
genetics;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
genetics;
Prognosis;
Reverse Transcriptase Polymerase Chain Reaction;
methods;
Translocation, Genetic;
genetics
- From:
Journal of Experimental Hematology
2006;14(2):228-231
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the usefulness of multiplex reverse transcription-polymerase chain reaction (multiplex RT-PCR) in detection of fusion genes associated with specific translocations in acute leukemia (AL) patients with normal karyotypes. 37 AL patients with normal karyotypes were analyzed by multiplex RT-PCR. The results showed that 4 types of fusion genes such as PML/RARA, AML1/ETO, CBFbeta/MYH11, BCR/ABL were detected in 8 (21.6%) patients by multiplex RT-PCR. In conclusion, multiplex RT-PCR is useful in detection of fusion genes associated with specific translocations in acute leukemia (AL) with normal karyotypes and it would refine the karyotype analysis. When the normal karyotypes were detected in acute leukemia patients by conventional cytogenetic method, the multiplex RT-PCR should be performed for them.
