Analysis of DEK-CAN fusion gene expression in acute myeloid leukemia patients with 6; 9 chromosome translocation.
- Author:
Ya-Lun WANG
1
;
Tong WANG
;
Feng XU
;
Yan GANG
;
Jie WANG
Author Information
1. Department of Biochemistry and Molecular Biology, Shenyang Medical College, Shenyang 110034, China.
- Publication Type:Journal Article
- MeSH:
Acute Disease;
Adolescent;
Adult;
Child;
Chromosomes, Human, Pair 6;
Chromosomes, Human, Pair 9;
Female;
Humans;
Leukemia, Myeloid;
genetics;
Male;
Oncogene Proteins, Fusion;
biosynthesis;
genetics;
Translocation, Genetic;
genetics
- From:
Journal of Experimental Hematology
2006;14(2):232-236
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the relationship of 6; 9 chromosome translocation with DEK-CAN fusion gene expression in patients with acute myeloid leukemia (AML) and its clinical significance. Chromosome specimens were prepared by routine method after short-term culture of bone marrow cells; karyotype analysis was performed by R banding technique; the expression of fusion gene DEK-CAN was analyzed by RT-nested-PCR in mononuclear cells of bone marrow or peripheral blood of 4 AML patients, for 3 patients received allo-BMT out of 4 patients the dynamic follow-up was performed. The results indicated that t (6; 9) (p23; q34) was confirmed by chromosome karyotype analysis in the four AML patients. The DEK-CAN fusion gene was found during in all four de novo, relapsed and CR patients (100%). And the expression of DEK-CAN fusion gene enhanced apparently in de novo and relapsed patients, and weakened in CR patient. DEK-CAN mRNA was found in the three patients during 1-24 months after allo-BMT. Clinical data showed 2 patients relapsed and died after CR for 1-24 months; the other two patients received allo-BMT got CR and still survive. It is concluded that DEK-CAN fusion gene is the molecular basis in pathogenesis of AML. The detection of DEK-CAN fusion gene is significant for diagnosis of AML, evaluation of curative effect, and predication of prognosis.
