Biological characteristics and ability of supporting hematopoiesis of bone marrow mesenchymal stem cells pre- and post-cryop-reservation.
- Author:
Zhi-Gang ZHAO
1
;
Xiao-Qiong TANG
;
Wei-Ming LI
;
Yong YOU
;
Ping ZOU
Author Information
1. Department of Hematology, Union Hospital, Tongji Medical College, Huazhong Univercity of Science and Technology, Wuhan 430022, China.
- Publication Type:Journal Article
- MeSH:
Blood Preservation;
methods;
Bone Marrow Cells;
cytology;
Cell Proliferation;
drug effects;
Colony-Forming Units Assay;
Cryopreservation;
methods;
Cryoprotective Agents;
pharmacology;
Dimethyl Sulfoxide;
pharmacology;
Hematopoiesis;
physiology;
Humans;
Mesenchymal Stromal Cells;
cytology;
physiology
- From:
Journal of Experimental Hematology
2006;14(2):304-307
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to observe the biological characteristics of cryopreserved bone marrow mesenchymal stem cell (MSC) and to examine their abilities to support in vitro hematopoiesis. Bone marrow MSC were cryopreserved in -196 degrees C liquid nitrogen for 4 weeks (short term) and 9-15 months (medium term) with IMDM containing 10% DMSO, 40% fetal calf serum as cryoprotectant. The viability, proliferation, immunophenotype, in vitro differentiation and ability of supporting hematopoiesis of thawed MSC were investigated and compared with these of pre-cryopreserved MSC. The results showed that the cell viability were (93 +/- 2.51)% and (90 +/- 3.75)% for MSC cryopreserved as long as 4 weeks or 9-15 months respectively. However, there were no changes detected, as compared with pre-cryopreserved MSC in immunophenotype, abilities of proliferation and supporting colony forming of CFU-GM, CFU-E and CFU-GEMM. It is concluded that bone marrow-derived MSC can be stored in liquid nitrogen for short-term (4 weeks) or medium-term (9-15 months) without changes of abilities of proliferation, differentiation and hematopoiesis support.
