Establishment of delta block matching technique.
- Author:
Qin-Feng LÜ
1
;
Wei ZHANG
;
Fa-Ming ZHU
;
Li-Xing YAN
Author Information
1. Blood Center of Zhejiang Province, Key Laboratory of Hematologic Safety Research, Ministry of Health, Hangzhou 310006, China.
- Publication Type:Journal Article
- MeSH:
HLA-A Antigens;
genetics;
immunology;
HLA-B Antigens;
genetics;
immunology;
HLA-DQ Antigens;
genetics;
immunology;
HLA-DR Antigens;
genetics;
immunology;
HLA-DRB1 Chains;
Hematopoietic Stem Cell Transplantation;
Histocompatibility Testing;
methods;
Humans
- From:
Journal of Experimental Hematology
2006;14(2):366-368
- CountryChina
- Language:Chinese
-
Abstract:
To establish delta block HLA-matching technique, DNA was extracted from whole blood by salting-out method, delta block was amplified by polymerase chain reaction (PCR), and PCR product was detected by GeneScan. The results showed that delta block had polymorphism in 104 samples without sibship of the Han people from Zhejiang province. The range of DNA fragment length was 81-393 bp and could be divided into 4 groups: 81-118 bp, 140-175 bp, 217-301 bp, 340-393 bp. The numbers of DNA fragments were 6-32. It is concluded that the method of delta block matching is reliable and can be applied to select donors for the patients to be transplanted. It is the first time to get delta block data of the Han people in China.
