Detection of pim-1 mRNA in prostate cancer diagnosis.
- Author:
Hui-chan HE
1
;
Xue-cheng BI
;
Qi-shan DAI
;
Shao-sheng WANG
;
Hong-ai WEI
;
Wei-de ZHONG
;
Wen-hua LIU
;
Fu-neng JIANG
;
Liang-shi LIU
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Humans; Male; Middle Aged; Polymerase Chain Reaction; Prostate; metabolism; Prostatic Hyperplasia; metabolism; Prostatic Neoplasms; diagnosis; metabolism; Proto-Oncogene Proteins c-pim-1; genetics; RNA, Messenger; analysis; Sensitivity and Specificity
- From: Chinese Medical Journal 2007;120(17):1491-1493
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDPim-1 plays an important role in the apoptosis, proliferation, differentiation of cancer cells and progression of cancer. In this study we detected the expression of pim-1 mRNA in normal prostate, benign prostatic hyperplasia (BPH), and prostate cancer (PCa) and explored its diagnostic value for PCa.
METHODSThe prostate tissues were collected from 23 patients with PCa, 37 patients with BPH, and 3 healthy volunteers. Pim-1 mRNA expression levels in these samples were determined by the quantitative real-time PCR (QRT-PCR). The differences of expression were calculated based on a standard curve.
RESULTSThe ratio of pim-1 mRNA to beta-actin in the normal prostate, BPH, and PCa were 1.05 +/- 0.04, 2.57 +/- 0.74 and 4.45 +/-0.63, respectively. The differences among PCa, BPH and NT were significant (P < 0.05, respectively).
CONCLUSIONDetecting pim-1 mRNA expression by QRT-PCR provides a reliable metric for the diagnosis of PCa.
