Detection of an NA gene molecular marker in H7N9 subtype avian influenza viruses by pyrosequencing.
- Author:
Yong-Gang ZHAO
;
Hua-Lei LIU
;
Jing-Jing WANG
;
Dong-Xia ZHENG
;
Yun-Ling ZHAO
;
Sheng-Qiang GE
;
Zhi-Liang WANG
- Publication Type:Journal Article
- MeSH:
Animals;
Base Sequence;
Birds;
Chickens;
High-Throughput Nucleotide Sequencing;
methods;
Influenza A Virus, H7N9 Subtype;
classification;
enzymology;
isolation & purification;
Influenza in Birds;
virology;
Molecular Sequence Data;
Neuraminidase;
genetics;
Phylogeny;
Poultry Diseases;
virology;
Viral Proteins;
genetics
- From:
Chinese Journal of Virology
2014;30(4):369-374
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to establish a method for the detection and identification of H7N9 avian influenza viruses based on the NA gene by pyrosequencing. According to the published NA gene sequences of the avian influenza A (H7N9) virus, a 15-nt deletion was found in the NA gene of H7N9 avian influenza viruses. The 15-nt deletion of the NA gene was targeted as the molecular marker for the rapid detection and identification of H7N9 avian influenza viruses by pyrosequencing. Three H7N9 avian influenza virus isolates underwent pyrosequencing using the same assay, and were proven to have the same 15-nt deletion. Pyrosequencing technology based on the NA gene molecular marker can be used to identify H7N9 avian influenza viruses.
