Preliminary study of a universal vaccine based on the HA2 protein of the H5N1 influenza virus.
- Author:
Li XIN
1
;
Xing-Yu YANG
;
Zai-Jiang YU
;
Hong BO
;
Jian-Fang ZHOU
;
Kun QIN
;
Yue-Long SHU
Author Information
1. World Health organization Collaborating Center for Reference and Research on Influenza, Chinese Center for Disease Control and Prevention, Beijing, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Female;
Hemagglutinin Glycoproteins, Influenza Virus;
immunology;
Influenza A Virus, H5N1 Subtype;
immunology;
Influenza Vaccines;
immunology;
Interferon-gamma;
biosynthesis;
Lung;
pathology;
Mice;
Mice, Inbred BALB C;
Molecular Sequence Data
- From:
Chinese Journal of Virology
2014;30(5):521-528
- CountryChina
- Language:Chinese
-
Abstract:
Fragments encoding amino acids 76-130 in the linear conserved region (LCR) of A/Hubei/1/2010 (H5N1) HA2 was fused to hepatitis B core antigen (HBc) to generate a LCR-HBe virus-like particle (VLP). Results showed that the fusion protein of LCR-HBc was highly expressed in this prokaryotic expression system. The purified LCR-HBc particle stimulated high levels of IgG production in mice with a titer of > 1:12 800, and provided 50% cross-protection against lethal challenge by H1N1 viruses.
