Pathogenic spectrum of enteroviruses associated with hand, foot and mouth disease by a GeXP™-based multiplex reverse transcription-PCR assay in Jinan, China, 2009-2012.
- Author:
Heng-Yun GUAN
1
;
Meng-Jie YANG
;
Lan-Zheng LIU
;
Ji WANG
;
Guo-Liang YANG
;
Wen ZHANG
;
Xue-Jun MA
;
Chun-Rong WANG
Author Information
1. Jinan Municipal Center for Disease Control and Prevention, Jinan, China.
- Publication Type:Journal Article
- MeSH:
Child;
Child, Preschool;
China;
Enterovirus;
genetics;
pathogenicity;
Hand, Foot and Mouth Disease;
virology;
Humans;
Infant;
Multiplex Polymerase Chain Reaction;
methods;
Time Factors
- From:
Chinese Journal of Virology
2014;30(5):567-571
- CountryChina
- Language:Chinese
-
Abstract:
We investigated the pathogenic spectrum of enteroviruses associated with hand, foot and mouth disease (HFMD) in Jinan, China. A total of 274 specimens with a clinical diagnosis of HFMD in Jinan from 2009 to June 2012 were used. A GenomeLab™ (GeXP)-based multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay was employed to simultaneously detect 15 serotypes of human enteroviruses: human enterovirus (EV)71; coxsackievirus A (CVA)16, 4, 5, 6, 9, and 10; CVB1, 3 and 5; echovirus (Echo) 6, 7, 11, 13 and 19. Results showed that all samples were enterovirus-positive, with the most common serotypes being EV71 (25.18%) and CVA16 (16.06%), followed by CVA10 (14.23%), CVA6 (7.30%), CVB1 (1.09%), Echo6 (0.73%), CVA9 (0.36%), CVB3 (0.36%) and co-infections (5.11%). CVA10 and CVA6 had the third and fourth highest prevalence of pathogens for HFMD, respec- tively. The most prevalent season for CVA10 was from April to August, with a peak in April; for CVA6 it was from April to August, with a peak in June. This is the first report of the pathogenic spectrum of en- teroviruses associated with HFMD in Jinan using the GeXP-based multiplex RT-PCR assay. These data will provide the scientific evidence for the prevention and control of epidemics, as well as therapy for HFMD patients.
