Research methods in protein-protein and protein-nucleic acid interactions and application in the study of human enterovirus A71.
- Author:
Zhi-Xiao ZHANG
1
;
Ying ZHENG
;
Ting-Song HU
;
Qi-Han LI
;
Quan-Shui FAN
Author Information
1. enter for Disease Control and Prevention, Chengdu Military Region, Kunming, China.
- Publication Type:Journal Article
- MeSH:
Electrophoretic Mobility Shift Assay;
Enterovirus A, Human;
chemistry;
genetics;
metabolism;
Fluorescence Resonance Energy Transfer;
RNA, Viral;
metabolism;
Two-Hybrid System Techniques;
Viral Proteins;
metabolism
- From:
Chinese Journal of Virology
2014;30(5):587-593
- CountryChina
- Language:Chinese
-
Abstract:
Methods for analyses of protein-protein interactions include: yeast two hybrid (Y2H), phage dis- play (PD), co-immunoprecipitation (Co-IP), glutathione S-transferase pull-down (GST pull-down), cellular co-localization, far-western blotting, virus overlay protein binding assay (VOPBA), surface plasmon resonance (SPR), and fluorescence resonance energy transfer (FRET). Technologies for the detection of protein-nucleic acid interactions include: yeast one hybrid (Y1H), chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assay (EMSA), Southwestern blotting, reporter gene, Co-IP, GST pull-down, and PD. These methods are often used in the study of the human enterovirus A71 (EV-A71) by our research team. Reviews in the Chinese literature in this field are lacking, so we reviewed applications of these methods in the study of EV-A71. This review may impart important knowledge in the research of other viruses with regard to protein-protein and protein-nucleic acid interactions.
