Analyses of Binding Profiles of the GII. 12 Norovirus with Human Histo-blood Group Antigens.
- Author:
Miao JIN
;
Kena CHEN
;
Jingdong SONG
;
Huiying LI
;
Qing ZHANG
;
Xiangyu KONG
;
Na LIU
;
Zhaojun DUAN
- Publication Type:Journal Article
- MeSH:
Animals;
Blood Group Antigens;
metabolism;
Caliciviridae Infections;
metabolism;
virology;
Female;
Gastroenteritis;
metabolism;
virology;
Genotype;
Humans;
Mice;
Mice, Inbred BALB C;
Norovirus;
genetics;
metabolism;
Protein Binding;
Receptors, Virus;
metabolism;
Viral Proteins;
genetics;
metabolism
- From:
Chinese Journal of Virology
2015;31(2):164-169
- CountryChina
- Language:Chinese
-
Abstract:
Interactions between noroviruses (NoVs) and the receptors of histo-blood group antigens (HB-GAs) affect the infectivity and host susceptibility of NoVs. We elucidated the binding profile of a GII. 12 NoV to HBGAs. First, we synthesized the P domain sequence of the GII. 12 NoV strain Pune (GenBank accession number EU921353). Protein of the P domain was expressed in a prokaryotic system. Formation of the P particle was monitored by gel-filtration chromatography. Antiserum was prepared by immunization of mice with GII. 12 P particles. The binding profile of the GII. 12 NoV Pune strain was determined by binding of the P particle with a panel of saliva samples with various known HBGAs phenotypes. The GII. 12 NoV was bound strongly to saliva samples of subjects with B and AB types and weakly to A, O secretor, and non-secretor saliva samples, suggesting higher affinity with B antigen by GII. 12 NoV. These results were consistent with those determined by a previous crystallography study of GII. 12 NoV. These data suggested that individuals with B and AB blood types may be more susceptible to infection by GII. 12 NoV compared with those with other blood types. Our findings may provide a basis for the prevention and control of an epidemic of GII. 12 NoV.
