Cloning and Sequence Analyses of Genome of Swine Hepatitis E Virus(HEV) HN-JY40 Strains Isolated from Henan Province.
- Author:
Xiaoxia ZHANG
;
Qin ZHANG
;
Zhenpu LIANG
;
Feng XU
;
Xinfeng SHAO
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Base Sequence;
China;
Cloning, Molecular;
Genome, Viral;
Hepatitis E;
veterinary;
virology;
Hepatitis E virus;
classification;
genetics;
isolation & purification;
Humans;
Molecular Sequence Data;
Open Reading Frames;
Phylogeny;
Sequence Homology, Amino Acid;
Swine;
Swine Diseases;
virology
- From:
Chinese Journal of Virology
2015;31(3):231-238
- CountryChina
- Language:Chinese
-
Abstract:
In the present study, the genomic sequence characteristics of HN-JY40 strains of the hepatitis E virus (HEV) isolated from pigs in Henan Province, China, were analyzed and the evolutionary relationship between HN-JY40 and other sequenced strains examined. The whole genome of HN-JY40 was sequenced and analyzed by reverse transcription-polymerase chain reaction, 3' rapid amplification of cDNA ends (3' RACE) and 5' RACE. Bioinformatic analyses were carried out with Megalign, Expasy, clustal x, and MEGA 4 software. The genome of HN-JY40 was 7 223 bp in size upon removal of polyA sequences. Sizes were 9 bp and 69 bp at 5' and 3' noncoding regions, respectively. The genome of HN-JY40 was predicted to contain three open reading frames (ORFs): ORF1 (5 124 bp) encoding 1 707 amino acids; ORF2 (2 025 bp) encoding 674 amino acids; ORF3 (345 bp) encoding 114 amino acids. Phylogenetic-tree analyses indicated that HN-JY40 is a typical type-IV virus that belongs to a new subgenotype of HEV genotype 4. We sequenced and analyzed the whole genome of HN-JY40. This strategy elicited the genomic characteristics of the HEV isolated from pigs in Henan Province as well as the evolutionary relationships between HN- JY40 and other HEV isolates from pigs. We revealed that the ORF1 of HN-JY40 (153-432 nt) and human HK 104-2004 had high similarity, which offers molecular evidence for uncovering the interspecies transmission of the HEV.
