Molecular Identification and Phylogenetic Analyses of Coxsackievirus A24v Causing an Outbreak of Acute Hemorrhagic Conjunctivitis in Jiangxi, China, in 2010.
- Author:
Dongmei YAN
;
Ying XIONG
;
Yang ZHANG
;
Qiai YANG
;
Shuxia ZHANG
;
Tian GONG
;
Tian ZHU
;
Donavan WANG
;
Hui ZHU
;
Wenbo XU
- Publication Type:Journal Article
- MeSH:
China;
epidemiology;
Conjunctivitis, Acute Hemorrhagic;
epidemiology;
virology;
Coxsackievirus Infections;
epidemiology;
virology;
Disease Outbreaks;
Enterovirus C, Human;
classification;
genetics;
isolation & purification;
Genotype;
Humans;
Molecular Sequence Data;
Phylogeny;
Viral Proteins;
genetics
- From:
Chinese Journal of Virology
2015;31(3):251-257
- CountryChina
- Language:Chinese
-
Abstract:
To identify the cause of an outbreak of acute hemorrhagic conjunctivitis (AHC) in Jiangxi (China) in 2010, 20 eye conjunctival swabs were first collected from AHC patients. Then, viruses were isola- ted and tested for human enterovirus 70, coxsackievirus A24 variant (CV-A24v) and adenovirus using the polymerase chain reaction. All CV-A24v isolates underwent sequencing of 3C and VP1 coding regions. Then, a phylogenetic tree was constructed for Jiangxi CV-A24v and worldwide CV-A24v based on,3C and VP1 regions, respectively. Ten out of 20 specimens were positive for CV-A24v, implying that the outbreak was caused by CV-A24v. The phylogenetic tree based on the 3C region showed that Jiangxi CV- A24v belonged to cluster 5 in genotype IV (GIV-C5) with strains isolated throughout the world after 2010, and were divided further into A and B lineages. Phylogenetic analyses of the VP1 region showed that all of the worldwide CV-A24v strains isolated after 2000 could be divided into five groups (1-5). Jiangxi CV-A24v was classified into group 5 and also divided further into A and B lineages upon analyses of the 3C region. These data suggested that CV-A24v causing AHC outbreaks in China in 2010 belonged to GIV-C3 and GIV-C5. At least two transmission lineages were circulated in Jiangxi in 2010. The classification of CV-A24v isolated after 2010 worldwide using the phylogenetic tree based on the VP1 region was almost consistent with that based on the 3C region and also had significant chronological clustering.
