The effect of immobilized laminin on titanium-oxide films to the behavior of human umbilical vein endothelial cells.
- Author:
Shengnan GE
1
;
Junying CHEN
;
Nan HUANG
Author Information
1. School of Materials Science and Engineering, Southwest Jiaotong University, Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Chengdu 610031, China.
- Publication Type:Journal Article
- MeSH:
Cell Proliferation;
Cells, Cultured;
Coated Materials, Biocompatible;
pharmacology;
Endothelial Cells;
cytology;
Humans;
Immobilized Proteins;
Laminin;
chemistry;
Titanium;
chemistry;
pharmacology;
Umbilical Veins;
cytology
- From:
Journal of Biomedical Engineering
2009;26(1):97-100
- CountryChina
- Language:Chinese
-
Abstract:
In this study, Ti-O films were synthesized using magnetron sputtering, and were pretreated using NaOH solution for improving surface activity from hydroxyl. The laminin(LN) biomacromolecule was further immobilized to the surface through an anminosilane linker. The surface characteristics of these samples were analyzed by Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy, Atomic Force Microscopy and the contact angle method. Finally, human umbilical vein endothelial cells (HUVEC) were in vitro seeded to the modified and unmodified Ti-O films surface for evaluating the cell compatibility. Survey results suggested that the functional group of hydroxyl was presented onto Ti-O film surface after being pretreated, and laminin could be covalently immobilized to Ti-O film surface by anminosilane linker. The in vitro cell culture results reveal that the biological behaviors of ECs on biochemical modified Ti-O film surface are excellent. The adherence, growth and proliferation of ECs on laminin-immobilized surface were obviously improved when compared to control one. It implies that the laminin immobilizing is helpful to increasing the endothelialization of Ti-O films.
