An experimental study on the cytokine expression of macrophage influenced by biomaterials.
- Author:
Guicai LI
1
;
Ping YANG
;
Jing WANG
;
Ansha ZHAO
;
Nan HUANG
Author Information
1. Key Lab. of Advanced Materials Technologies of Education Ministry, Key Lab. of Artificial Organ Surface Engineering of Sichuan, School of Material and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Biocompatible Materials;
pharmacology;
Cells, Cultured;
Chemokine CCL2;
metabolism;
Macrophages;
cytology;
metabolism;
Male;
Materials Testing;
methods;
Nitric Oxide;
metabolism;
Rats;
Rats, Sprague-Dawley;
Stainless Steel;
pharmacology;
Titanium;
pharmacology
- From:
Journal of Biomedical Engineering
2009;26(1):105-109
- CountryChina
- Language:Chinese
-
Abstract:
This experiment was designed to investigate the influence of two biomaterials, titanium oxide (Ti-O) and stainless steel (SS), on the cytokine expression of macrophage, and further, to evaluate their biocompatibility. After being co-cultured with Ti-O and SS for 72 h, the cell number and morphology of macrophages attached on materials were detected by fluorescent microscope and SEM. Nitride oxide (NO) and monocyte chemoattractant protein 1 (MCP-1) released by the macrophages co-cultured with different materials were also examined and compared. We found that the cell number of macrophages attached to Ti-O was smaller than that attached to SS. The levels of NO and MCP-1 released by the macrophages co-cultured with Ti-O were lower when compared with those released by macrophages co-cultured with SS. These results demonstrate that Ti-O has better biocompatibility than does SS.
