Effect of regenerated silk fibroin film on transcription and expression of vascular endothelial growth factor gene.
- Author:
Quan HUANG
1
;
Jicheng YANG
;
Jingcheng MIAO
;
Chunyu LIU
;
Yufeng XIE
;
Weihua SHENG
;
Mingzhong LI
Author Information
1. Medical School, Soochow University, Suzhou 215123, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Biocompatible Materials;
pharmacology;
Cell Line;
Endothelial Cells;
cytology;
metabolism;
Fibroins;
pharmacology;
Humans;
Silk;
pharmacology;
Transcription, Genetic;
Vascular Endothelial Growth Factor A;
genetics;
metabolism
- From:
Journal of Biomedical Engineering
2009;26(1):110-115
- CountryChina
- Language:Chinese
-
Abstract:
As a biomaterial to be used for reparation in the case of trauma, the silk fibroin, particularly its effect on the transcription and expression of VEGF gene, is a concern. In this study, the ECV304 cell's growth shape and growth curve on the regenerated silk fibroin film were observed, and its VEGF secretion level was measured by ELISA test. It was found that the regenerated silk fibroin film did not interfere with ECV304 cell's growth and function. The L929 cell transfected with human VEGF gene grew on the regenerated silk fibroin film; the real-time quantitative RT-PCR method and ELISA test were used for detecting the transcription and expression of VEGF gene. The results showed the regenerated silk fibroin film did not interfere with the transcription and expression of VEGF gene. Therefore, the regenerated silk fibroin film is a safe biomaterial for inducing vascularization with no untoward effect on the reparation of trauma.
