Expression of Bax, Bcl-2 and P53 in the HeLa apoptosis induced by co-immobilized cytokines.
- Author:
Yanqing GUAN
1
;
Lili QIU
;
Wenwen WANG
Author Information
1. College of Life Science, South China Normal University, Guangzhou 510631, China. gyq69@163.com
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
HeLa Cells;
Humans;
Immobilized Proteins;
pharmacology;
Interferon-gamma;
pharmacology;
Photochemistry;
methods;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Tumor Necrosis Factor-alpha;
pharmacology;
Tumor Suppressor Protein p53;
metabolism;
bcl-2-Associated X Protein;
genetics;
metabolism
- From:
Journal of Biomedical Engineering
2009;26(1):138-143
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to evaluate the changes in the expression of Bax, Bcl-2 and P53 when HeLa cells were induced by free cytokines or co-immobilized cytokines. The cells were induced for 24 hrs,72 hrs, 120 hrs and 168 hrs. Then, the expression of Bax, Bcl-2 and P53 was observed by immunohistochemistry. The average optic density of reaction products was tested by image analysis. Lastly, data were analyzed statistically. After the HeLa cells were induced for 120 hrs, the average optic denisity of Bcl-2 was much decreased. However, the average optic Bax was much increased. The average optic density of P53 also increased with the increase of time. The results suggest that HeLa apoptosis was induced by tumor necrosis factor-a and interferon -gamma, and the increasing expression of P53 may induce the expression of Bax and prevent the expression of Bcl-2, via the mitochondrial induction of cell apoptosis.
