Experimental study on prevention of postsurgical adhesions by using O-carboxymethylchitosan solution after an abdominal operation.
- Author:
Shirong PAN
1
;
Jiacong MO
;
Huanling ZHENG
;
Wu YI
;
Daquan HUANG
;
Bi WANG
Author Information
1. The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China. gzpshr@163.com
- Publication Type:Journal Article
- MeSH:
Abdomen;
surgery;
Animals;
Chitosan;
analogs & derivatives;
therapeutic use;
Female;
Intestinal Diseases;
prevention & control;
Male;
Postoperative Complications;
prevention & control;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Tissue Adhesions;
prevention & control
- From:
Journal of Biomedical Engineering
2009;26(2):347-359
- CountryChina
- Language:Chinese
-
Abstract:
O-Carboxymethylchitosan (O-CMC) in 1000 g batch was prepared from chitin as starting material and its chemical structure was confirmed by analysis of IR and NMR. O-CMC solution, sodium hyaluronate (HA) solution and physiological saline were used in Sprague-Dawley rat model for prevention of postsurgical adhesions; after 7 days of an abdominal operation, the 3 groups were evaluated according to Belluco standard, the mean scores of O-CMC group, HA group and physiological saline group were 2.5 +/- 3.1, 3.3 +/- 3.6 and 10.3 +/- 1.0, respectively. Histological inspection showed that in O-CMC group, mesothelial cells on peritonaeum or cecum surfaces were almost restored; in HA group the injured surface of peritonaeum was mostly repaired, but in physiological saline group the injured surface of cecum was just a little repaired; there were extensive adhesions between peritonaeum and cecum, and inflammatory response was quite serious. Experimental results indicated that O-CMC and HA had excellent efficiency and O-CMC was slightly better than HA for the prevention of postsurgical adhesions.
