Fluorescent linear labelling method for biochip applications.
- Author:
Dapeng DING
1
;
Wenli MA
;
Ying ZHANG
;
Rong SHI
;
Jueyu ZHOU
;
Wenling ZHENG
Author Information
1. Institute of Genetic Engineering, Nanfang Medical University, Guangzhou 510515, China.
- Publication Type:Journal Article
- MeSH:
Fluorescent Dyes;
chemistry;
Humans;
Oligonucleotide Array Sequence Analysis;
methods;
Staining and Labeling;
methods
- From:
Journal of Biomedical Engineering
2009;26(2):405-412
- CountryChina
- Language:Chinese
-
Abstract:
Fluorescent Linear Labelling technique is an effective, single-tube and linear amplification method. The sample cDNA was synthesized from total RNA, and was labelled antisense cRNA from double-stranded cDNA with T7RNA polymerase; it can be used for hybridization to oligonucleotide biochip. Fluorescent Linear Labelling Method can result in 50- to 100-fold higher degrees of amplification, and has been shown to retain information on transcript abundance, thus making it an efficient, robust technique for fluorescent labelling on biochip.
