Effects of Newcastle disease virus on the expression of survivin and cell cycle in human tongue squamous carcinoma TSCCa cells.
- Author:
Xue-qin WAN
1
;
Guo-yi DAI
;
Chang-wu DOU
;
Fu-ming TIAN
;
Min LONG
;
Bei-guo LONG
;
Xiao-juan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; physiology; Blotting, Western; Carcinoma, Squamous Cell; metabolism; pathology; virology; Cell Cycle; physiology; Cell Line, Tumor; Host-Pathogen Interactions; Humans; Inhibitor of Apoptosis Proteins; Microtubule-Associated Proteins; biosynthesis; genetics; Newcastle disease virus; physiology; Reverse Transcriptase Polymerase Chain Reaction; Tongue Neoplasms; metabolism; pathology; virology
- From: Journal of Southern Medical University 2008;28(4):558-561
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Newcastle disease virus (NDV) infection on the expression of survivin and cell cycle in human tongue squamous carcinoma TSCCa cells.
METHODSThe proliferation of TSCCa cells infected with NDV in vitro was evaluated by means of MTT assay, and survivin expression in the infected cells was detected using RT-PCR and Western blotting. Flow cytometry was performed to assess the changes in the cell apoptosis, cell cycle and cell proliferation index (PI) of the cells.
RESULTSNDV infection resulted in decreased survivin expression and increased apoptosis of TSCCa cells, with reduced cell percentage in G2/M and S phases and lowered PI of the cells, showing significant differences from those of the negative control cells (P<0.05).
CONCLUSIONNDV infection can inhibit survivin expression, affect the cell cycle of TSCCa cells and induce their apoptosis.
