Nuclear microarray combined with fluorescence in situ hybridization for detecting ALK gene translocation in paraffin-embedded anaplastic large cell lymphoma and its significance.
- Author:
Hui-ling LI
1
;
Hui-yong JIANG
;
Tian-hai JI
;
Hong-juan CHU
;
Fang LIU
;
Xiao-yan CHEN
;
Xin WANG
;
Gong ZHANG
;
Tong ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Child; Child, Preschool; Female; Humans; Immunohistochemistry; In Situ Hybridization, Fluorescence; methods; Lymphoma, Large-Cell, Anaplastic; enzymology; genetics; pathology; Male; Microarray Analysis; methods; Middle Aged; Paraffin Embedding; Protein-Tyrosine Kinases; genetics; Receptor Protein-Tyrosine Kinases; Reproducibility of Results; Translocation, Genetic; Young Adult
- From: Journal of Southern Medical University 2008;28(4):572-575
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the efficacy of nuclear microarray combined with fluorescence in situ hybridization (FISH) and immunohistochemistry in detecting ALK gene translocation and ALK fusion protein in anaplastic large cell lymphoma (ALCL).
METHODSALK gene translocation and ALK fusion protein in 17 paraffin-embedded ALCL specimens were detected using nuclear microarray combined with FISH and immunohistochemical straining, respectively.
RESULTSThe expression of ALK fusion protein was detected immunohistochemically with ALK antibody in 8 of the 17 specimens of systemic ALCL, including 4 with both nuclear and cytoplasmic positivity and 4 with only cytoplasmic positivity. Dual-color FISH identified 6 positive specimens, including the 4 specimens with both nuclear and cytoplasmic positivity as identified immunohistochemically, and 2 with immunohistochemical cytoplasmic positivity. FISH yielded negative results for the 2 specimens with immunohistochemical cytoplasmic positivity.
CONCLUSIONNuclear microarray combined with FISH eliminated the cytoplasmic interference of the results of conventional FISH and provides a high-throughput platform for clinical detection with greater specificity than immunohistochemistry.
