Changes of protein kinase C activity in experimental presyrinx state in rabbits.
- Author:
Guo-zhu SUN
1
;
Qing-shan HU
;
Qing-jun ZHANG
;
Zong-mao ZHAO
;
Geng-shen ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Kaolin; Male; Protein Kinase C; metabolism; Rabbits; Random Allocation; Spinal Cord; enzymology; Syringomyelia; chemically induced; enzymology
- From: Journal of Southern Medical University 2008;28(4):582-584
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes of protein kinase C (PKC) activity and its role in the development of presyrinx state in rabbits.
METHODSPresyrinx state was established in 56 rabbits by intra-cisternal injection of kaolin. At 1, 3, 7, 14, and 21 days after the injection, the water content in the upper cervical spinal cord was measured, its pathological changes observed microscopically and the PKC activity determined with substrate phosphorolysis kinase assay.
RESULTSSpinal cord edema occurred in rabbits one day after kaolin injection, with water content of (68.35-/+0.70)%, which increased to (72.70-/+0.88)% on day 3, reaching the peak level of (72.92-/+0.86)% on day 7, followed by gradual decline after 3 weeks [(70.03-/+0.77)%]. The membrane PKC activity increased from 5.67-/+0.26 pmol.mg(-1).min(-1) on day 1 after the injection to reach the peak level on day 7 (13.27-/+3.15 pmol.mg(-1).min(-1)), which was maintained till day 14 with subsequent decrease to 8.85-/+1.56 pmol.mg(-1).min(-1) on day 21. The cytoplasmic PKC activity showed changes of a reverse pattern.
CONCLUSIONIn rabbits with experimental presyrinx state, PKC translocation and activation is involved in ischemic spinal edema.
