Inhibitory effects of recombinant human endostatin on the metastatic potential and invasiveness of hepatocellular carcinoma HCCLM6 cells in vitro.
- Author:
Peng-hai ZHANG
1
;
Jing QIN
;
Ai-min LI
;
Xue-mei DING
;
Du YANG-FENG
;
Luo RONG-CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Carcinoma, Hepatocellular; pathology; physiopathology; Cell Adhesion; drug effects; Cell Line, Tumor; Cell Movement; drug effects; Cell Proliferation; drug effects; Cell Survival; drug effects; Dose-Response Relationship, Drug; Endostatins; genetics; pharmacology; Humans; Liver Neoplasms; pathology; physiopathology; Neoplasm Invasiveness; Neoplasm Metastasis; Recombinant Proteins; pharmacology
- From: Journal of Southern Medical University 2008;28(4):658-661
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibitory effects of recombinant human endostatin (rh-ES) on cell adhesion, metastatic potential and invasiveness of hepatocellular carcinoma HCCLM6 cells in vitro.
METHODSThe changes in the cell proliferation status of HCCLM6 cells treated with different concentrations of rh-Endostatin in vitro was measured with MTT assay, and their invasiveness and migration were assayed using transwell cell culture chamber. The cell adhesion assay was carried out on 96-well plate precoated with matrigel.
RESULTSRh-ES showed inhibitory effect against the proliferation of HCCLM6 cells after a 72-h treatment. The adhesion, metastatic potential and invasiveness of the cells were obviously inhibited by rh-Endostatin in a dose-dependent manner.
CONCLUSIONRh-ES inhibits the adhesion, invasiveness and migration of hepatocellular carcinoma cells in vitro, and the mechanism needs further investigation.
