Effect of Tongxinluo on the proliferation and differentiation of rat embryonic neural stem cells.
- Author:
Hai-shan JIANG
1
;
Qiu-shi LI
;
Bing-xun LU
;
Li-xin WANG
;
Rui-xue YIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; pharmacology; Embryonic Stem Cells; cytology; Female; Fluorescent Antibody Technique; Male; Neurons; cytology; Pregnancy; Rats; Rats, Sprague-Dawley
- From: Journal of Southern Medical University 2008;28(5):679-683
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Tongxinluo in on the proliferation and differentiation of rat embryonic neural stem cells (NSCs).
METHODSNSCs were isolated from 12- to 14-day SD rat embryo and treated with Tongxinluo at different doses, and the proliferation and differentiation of the cells were observed by immunofluorescence staining at different time points.
RESULTSThe ratio of embryonic NSCs labeled with nestin decreased soon after Tongxinluo treatment, but increased afterwards. Significant difference was noted in the number of cells labeled with beta-tubulin between Tongxinluo group and the control group 3 and 7 days after the treatment, and also between high-dose and low-dose Tongxinluo groups at 7 days.
CONCLUSIONTongxinluo can induce the proliferation and neuronal differentiation of rat embryonic NSCs, and the effect is related to the dose of Tongxinluo administered.
