Function of F10 gene, a novel hydatidiform mole-related gene: a preliminary study.
- Author:
Xiao-min CAO
1
;
Zhan-jun PANG
;
Song QUAN
;
Fu-qi XING
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Cell Proliferation; Epithelial Cells; metabolism; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Hydatidiform Mole; genetics; Hydatidiform Mole, Invasive; genetics; Lung Neoplasms; genetics; pathology; Oncogenes; genetics; Pregnancy; Transfection; Uterine Neoplasms; genetics
- From: Journal of Southern Medical University 2008;28(5):722-724
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the function of F10 gene, a novel hydaditiform mole-related gene.
METHODSA549 cell line was transfected with the F10 gene of forward or reverse sequence or with the empty vector, respectively. The cellular mRNA was extracted after 24 h of transfection to screen for the differentially expressed genes among the 3 transfected and the control cells using differential display-polymerase chain reaction (ddPCR).
RESULTSThe bands representing differentially expressed genes were amplified from the cells, and the products were linked to T-Vector for sequence analysis. Several genes were screened by Blasting and their expressions were confirmed by fluorescent quantitative PCR.
CONCLUSIONF10 gene is functionally related to cell proliferation and apoptosis.
