Development of a SPA-ELISA method for detecting anti-coronavirus IgG antibodies in serum samples from fulvous fruit bats.
- Author:
Jie ZHOU
1
;
Yu-xue LIAO
;
Zhong CHEN
;
Yu-chun LI
;
Lu-Lu GAO
;
Yi-xiong CHEN
;
Lian-gong CAI
;
Qing CHEN
;
Shou-yi YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Viral; blood; Chiroptera; virology; Coronavirus; immunology; isolation & purification; Enzyme-Linked Immunosorbent Assay; methods; Immunoglobulin G; blood
- From: Journal of Southern Medical University 2008;28(5):736-738
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an simple and sensitive method for detecting anti-coronavirus IgG antibodies in bat sera based on enzyme-linked immunosorbent assay (ELISA).
METHODSA commercial ELISA kit for detecting SARS-CoV antibody was modified for detecting coronavirus antibodies in bat serum samples. The second antibody in the kit was replaced with horseradish peroxidase-conjugated protein-A (HRP-SPA) based on the characteristics of binding between Staphylococcus aureus protein A (SPA) and mammal IgG Fc fragment. The sera of 55 fulvous fruit bats (Rousettus dasymallus) were tested using the SPA-ELISA.
RESULTSThe test results of the positive and negative controls in the kit and the serum samples from convalescent ;patient were consistent with expectation. Coronavirus antibody was detected in 2 out of the 55 bat serum samples. Serum neutralization test confirmed the validity of the SPA-ELISA method.
CONCLUSIONThis SPA-ELISA method is applicable for detecting coronavirus antibody in bat sera.
