Isolation and characterization of the first strain of Helicobacter hepaticus in China.
- Author:
Jing LI
1
;
Huan-jian LIN
;
Bo JIANG
;
Yang BAI
;
Di ZHANG
;
Ya-li ZHANG
;
Ji-de WANG
Author Information
1. Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Lijing1801@126.com
- Publication Type:Journal Article
- MeSH:
Animals;
China;
DNA, Bacterial;
analysis;
genetics;
Helicobacter hepaticus;
genetics;
isolation & purification;
ultrastructure;
Intestines;
microbiology;
Mice;
Mice, Inbred BALB C;
Microscopy, Electron, Scanning;
RNA, Ribosomal, 16S;
genetics
- From:
Journal of Southern Medical University
2008;28(5):843-845
- CountryChina
- Language:Chinese
-
Abstract:
Helicobacter hepaticus is nongastric helicobacter that can reside in the hepatobiliary and intestinal systems of many animal hosts, leading to proliferative hepatitis, hepatocellular carcinoma, typhlitis, and colonitis. In this study, the intestinal mucosa was isolated from BALB/c mice to prepare tissue homogenate and spread onto selective C jejuni blood agar plates for incubation in the presence of trimethoprim, vancomycin, and polymyxin at 37 degrees Celsius; under microaerobic conditions in vented jars containing 5% O2, 10%CO2, and 85% N2. The bacteria were identified morphologically and biochemically. Gene sequence analysis of the 16s rRNA confirmed the presence of Helicobacter hepaticus, and the success in isolating this bacteria may have significant implications for studies of nongastric helicobacter.
