High glucose suppresses ABCG1 expression by increasing oxidative stress and inducing nuclear factor-kappaB activation in vascular smooth muscle cells.
- Author:
Jia-Hong XUE
1
;
Zu-Yi YUAN
;
Yue WU
;
Yan ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: ATP Binding Cassette Transporter 1; ATP Binding Cassette Transporter, Sub-Family G, Member 1; ATP-Binding Cassette Transporters; biosynthesis; genetics; Aorta; cytology; Blotting, Western; Cells, Cultured; Dose-Response Relationship, Drug; Down-Regulation; Glucose; pharmacology; Humans; Muscle, Smooth, Vascular; cytology; drug effects; metabolism; NF-kappa B; metabolism; Oxidative Stress; drug effects; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: Journal of Southern Medical University 2008;28(6):933-937
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of high glucose in the expression of ATP-binding cassette (ABC) transporters A1 (ABCA1) and G1 (ABCG1) in human vascular smooth muscle cells (VSMCs) and its possible mechanisms.
METHODSVSMCs were incubated in the presence of glucose at the concentrations ranging from 5 to 30 mmol/L for 1 to 7 days, and real-time PCR and Western blotting were used to measure the mRNA and protein expressions of ABCA1 and ABCG1. The effects of cells pretreatment with antioxidant NAC (10 mmol/L) and nuclear factor-kappaB (NF-kappaB) inhibitors BAY 11-7085 (10 micromol/L) and TPCK (10 micromol/L) were also tested on ABCA1 and ABCG1 expressions.
RESULTSHigh glucose suppressed, in a time- and dose-dependent manner, ABCG1 expression in incubated human VSMCs, and this effect was abolished by pretreatment with the antioxidant and nuclear factor-kappaB (NF-kappaB) inhibitors, but ABCA1 expression was not significantly decreased in the presence of high glucose.
CONCLUSIONHigh glucose suppresses ABCG1 expression in human VSMCs possibly due to increased oxidative stress and NF-kappaB activation induced by high glucose.
