Determination of HLA-A, B, and DRB1 gene polymorphism in Monba ethnic group of Xizang Autonomous Region, China.
- Author:
Hong-bo ZHANG
1
;
Fang GAO
;
Long-li KANG
;
Sheng-bin LI
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; China; Ethnic Groups; classification; genetics; Gene Frequency; HLA Antigens; genetics; HLA-B Antigens; genetics; HLA-DR Antigens; genetics; HLA-DRB1 Chains; Humans; Phylogeny; Polymerase Chain Reaction; Polymorphism, Genetic; genetics
- From: Chinese Journal of Medical Genetics 2005;22(3):344-346
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the HLA-A, B, DRB1 allele polymorphism in Monba ethnic population of Xizang Autonomous Region.
METHODSHLA-A, B, DRB1, DNA types in 47 unrelated Monba ethnic healthy individuals from the Xizang Autonomous Region of China were analyzed by polymerase chain reaction-based reverse line-strip sequence specific oligonucleotide hybridization. Based on the HLA-DRB1 alleles frequencies of 11 other ethnic groups in China, a molecular phylogenetic tree was constructed by the Neighbor-Joining (NJ) method.
RESULTSTwenty-three alleles were detected for HLA-A, 39 for HLA-B, and 33 for HLA-DRB1. HLA-A*1101, A*2402, A*02011, A*0206, HLA-B*3802, B*4001, B*4002, B*51011, HLA-DRB1*12021, DRB1*0403, DRB1*0701, DRB1*1201 were the most common alleles. The most frequent alleles were HLA-A*1101(0.2128), HLA-B*3802(0.1064), and HLA-DRB1*12021(0.0851).
CONCLUSIONThe HLA loci are highly polymorphic in Monba population of Xizang Autonomous Region. Compared with other ethnic groups in China, the Monba ethnic group is close to Tibetan ethnic group.
