Association of TNFA polymorphisms with the outcomes of HBV infection.

Ying Liu; Xin-hui Guo; Te DU; Jun-hong LI; Xi-lin Zhu; Ji-rong Gao; Liang-ping LU; Chun-yan Gou; Zhuo LI; Hui LI

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Association of TNFA polymorphisms with the outcomes of HBV infection.

Author: Ying LIU ^{1
,

2} ; Xin-hui GUO ; Te DU ; Jun-hong LI ; Xi-lin ZHU ; Ji-rong GAO ; Liang-ping LU ; Chun-yan GOU ; Zhuo LI ; Hui LI
Author Information

1. Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
2. School of Basic Medicine, Peking Union Medical College, Beijing, 100005 PR China.
Publication Type:Journal Article
MeSH: Adult; Female; Gene Frequency; Genetic Predisposition to Disease; genetics; Genotype; Haplotypes; Hepatitis B; genetics; Humans; Male; Middle Aged; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Tumor Necrosis Factor-alpha; genetics; Young Adult
From: Chinese Journal of Medical Genetics 2005;22(4):406-410
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore whether the TNFA promoter single nucleotide polymorphisms (SNPs) are associated with the outcomes of hepatitis B virus(HBV) infection in Chinese Han population.
METHODSOne hundred and forty-eight self-limited HBV infection subjects and 207 chronic hepatitis B patients were recruited. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequence specific primer-PCR(PCR-SSP) were used to detect the SNPs of five sites in TNFA promoter (-238G/A, -308G/A, -857C/T, -863C/A, -1031T/C). The frequency distributions of genotypes and haplotypes in different groups were analyzed by EPI and EH programs.
RESULTSThe frequencies of -238GG genotype in chronic hepatitis B patients were significantly higher than that in self-limited infection subjects (P=0.02). The frequencies of -857TT genotype in chronic hepatitis B patients were clearly lower than that in self-limited infection subjects (P=0.02). Haplotypic frequencies of GGCCT (-238/-308/-857/-863/-1031) in chronic hepatitis B patients was significantly lower than that in self-limited infection subjects (P=0.03), and the frequencies of haplotype GGCAT or GGTAT in chronic hepatitis B patients were clearly higher than those in self-limited infection subjects (P=0.0001; P=0.004).
CONCLUSIONTNFA promoter polymorphisms are important host genetic factors affecting the outcomes of HBV infection.