Association of Xba I, Pvu II, and Bst U I polymorphisms of estrogen receptor-alpha gene with bone mass in men.
- Author:
Zhen-lin ZHANG
1
;
Yue-juan QIN
;
Jin-wei HE
;
Qi-ren HUANG
;
Yun-qiu HU
;
Miao LI
;
Yu-juan LIU
;
Hao ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Aged, 80 and over; Base Sequence; Bone Density; genetics; Estrogen Receptor alpha; genetics; Exons; genetics; Gene Frequency; genetics; Genotype; Humans; Male; Middle Aged; Molecular Sequence Data; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length
- From: Chinese Journal of Medical Genetics 2005;22(4):447-449
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the association of polymorphism in estrogen receptor-alpha (ER-alpha ) gene with bone mineral density(BMD) in men.
METHODSThe ER-alpha Xba I, Pvu II and Bst UI genotypes were determined by PCR-restriction fragment length polymorphism (RFLP) in 388 unrelated healthy men who were 46-80 years old and were of Han nationalities in Shanghai city. Bone mineral densities (BMD, g/cm(2)) at lumbar spines 1-4 (L(1-4)) and at any sites of proximal femur, including femoral neck (Neck), trochanter (Troch) and Ward's triangle (Ward's) were measured by duel-energy X-ray absorptiometry.
RESULTSThe frequencies distribution of Xba I and Pvu II alleles and genotypes in this cohort all followed the Hardy-Weinberg equilibrium. No Bst UI polymorphic site in ER-alpha gene was found in total samples. All subjects were of BB genotype. No significant association was found between Xba I genotype and BMD at any skeleton sites. The significant association was found between Pvu II genotype and BMD at L(1-4) and Ward's triangle site (P< 0.05). Compared against men with PP and pp genotype, men with Pp genotype had significantly higher mean BMD at L(1-4) and Ward's triangle site (P< 0.05).
CONCLUSIONThis study suggests that Bst UI polymorphism in ER-alpha gene may be absent or rare in Chinese Han population. Pvu II polymorphism possibly influences the loss of trabecular bone mass in old men.
